Over the course of the series, and with the significant support of several friends and role models who also struggle with LGBT+ issues, Shuichi comes to terms with her identity as a transgender girl. I'll use female pronouns to reflect this.
Shuichi certainly struggles with being accepted for her preferences, starting with her home life and her sister, Maho, who outspokenly opposes her transition and views it as a sickness. However, Shuichi isn't framed by the show as a joke or as an inherent outcast-- a fact that is almost surprising considering the strict norms of Japanese society.
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One close friend, Yoshino Takatsuki, is a transgender boy. Yoshino and Shuichi have known each other through elementary school, and continue to experience the growing pains of getting older together. He is the clearest parallel and complement to Shuichi's situation, and somewhat of a complicated love interest.
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A mentor figure appears in the form of Yuki Yoshida, an adult transsexual woman. She lives with her boyfriend after being rejected by her family, but has found a successful career running a gay bar. With her relevant life experience, she is able to offer advice to Shuichi and Yoshino.
Not everyone is friendly and compatible at first brush. Anna Suehiro, who plays an important role as Shuichi's first real relationship, is a girl who initially teases him for his cross-dressing, but the two soon develop genuine interest in each other. She comes to accept Shuichi as a person rather than necessarily as a boy or girl, gets to understand her feelings, and goes on a date with her while in feminine clothes, not minding if she is seen as a lesbian. Their relationship may not work out in the end, but it leaves an impact on them both.
One more noteworthy classmate, Chizuru Sarashina, is a girl with a very androgynous attitude, who shows up on the first day of school in a male uniform "on a whim," continuing to do so randomly throughout the year. Yoshino frequently envies Chi's freedom, wishing she could be so carefree about her own expression.
The series deals with the double standards of gender presentation: Chizuru and Yoshino each come to school in a male uniform without much consequence, but many people -- including Yoshino herself -- at some point criticize Shuichi for wanting to wear a female uniform in public. It also touches upon the background radiation that contributes to the gendered society: Constant references appear in casual conversations as to what "girls should do" and what "boys should do." Even the title of the first episode, referencing a common nursery rhyme, asks the question, "What are little girls made of?"
Utilizing the character-centered nature of the slice-of-life genre to its fullest, despite the slow plot, relationships and feelings take center stage. To create the context, the majority of the plotline revolves around Shuichi's middle-school class preparing to produce and perform a "gender-bender" play, one which purposely casts males in female roles and vice versa. Somewhat fittingly, the play they choose to present is based on Romeo and Juliet. Shuichi helps write the script, re-envisioning the story to reflect the themes of the actors' own gender insecurities. As many characters are being given their first major chance to publicly play a different gendered role, this setting offers a significant frame for their exploration.
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Many of the characters are able to "pass" attractively as both genders whose roles they explore, due to a sense of natural physical androgyny. Natural androgyny is often used as a convenient handwave to justify gender-bending characters, but in this show they are also shown to run into physical challenges when the effects of puberty hit. Yoshino becomes anxious over developing breasts and the pressure to wear a bra, while Shuichi's deeper voice and changing body begins to makes her less comfortable in her femininity... but, once again, they have support to encourage them through it.
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This review was suggested by Tumblr user toyherb, whom I thank for introducing me to such a beautiful show!
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